pFG001
(Plasmid
#62816)
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PurposeBacterial constitutive gRNA expression plasmid
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFG001
- Total vector size (bp) 3677
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namegRNA
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gRNA/shRNA sequenceATGCTCGATGAGTTTTTCTANGG
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SpeciesSynthetic; Streptococcus pyogenes
- Promoter PN25
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AGTATATTTTAGATGAAGATTATTTCTTAA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFG001 was a gift from Sébastien Rodrigue (Addgene plasmid # 62816 ; http://n2t.net/addgene:62816 ; RRID:Addgene_62816)
Map uploaded by the depositor.
