pcmCherry 3.1(-)
(Plasmid
#62803)
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Purpose(Empty Backbone) Modified pcDNA3.1(-), with Neomycin resistance cassette replaced by mCherry
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62803 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(-) (modified)
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Backbone manufacturerMinczuk Laboratory
- Backbone size (bp) 5343
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Modifications to backboneComplete pcDNA3.1(-) with intact multiple cloning site. Neomycin resistance cassette replaced with mCherry, using Gibson Assembly.
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Vector typeMammalian Expression
- Promoter CMV
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Selectable markersmCherry fluorescence
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcmCherry 3.1(-) was a gift from Michal Minczuk (Addgene plasmid # 62803 ; http://n2t.net/addgene:62803 ; RRID:Addgene_62803)
