pcDNA3-HA-BRAT1
(Plasmid
#62802)
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PurposeExpresses human BRAT1 with an N-terminal HA tag in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBRAT1
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Alt nameBRCA1-associated ATM activator 1
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SpeciesH. sapiens (human)
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Entrez GeneBRAT1 (a.k.a. BAAT1, C7orf27, NEDCAS, RMFSL)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-HA-BRAT1 was a gift from Toru Ouchi (Addgene plasmid # 62802 ; http://n2t.net/addgene:62802 ; RRID:Addgene_62802) -
For your References section:
BRAT1 deficiency causes increased glucose metabolism and mitochondrial malfunction. So EY, Ouchi T. BMC Cancer. 2014 Jul 29;14:548. doi: 10.1186/1471-2407-14-548. 10.1186/1471-2407-14-548 PubMed 25070371