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Addgene

pcDNA3.1(-)_mtZFN(-)2G
(Plasmid #62799)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62799 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5427
  • Total vector size (bp) 6625
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mtZFN(-)2G
  • Species
    Synthetic
  • Insert Size (bp)
    1266
  • Promoter CMV
  • Tag / Fusion Protein
    • Internal FLAG tag immediately after F1B mitochondrial targeting sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(-)_mtZFN(-)2G was a gift from Michal Minczuk (Addgene plasmid # 62799 ; http://n2t.net/addgene:62799 ; RRID:Addgene_62799)

  • For your References section:

    Mitochondrially targeted ZFNs for selective degradation of pathogenic mitochondrial genomes bearing large-scale deletions or point mutations. Gammage PA, Rorbach J, Vincent AI, Rebar EJ, Minczuk M. EMBO Mol Med. 2014 Apr;6(4):458-66. doi: 10.1002/emmm.201303672. Epub 2014 Feb 24. 10.1002/emmm.201303672 PubMed 24567072
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