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Addgene

pARD (pKD-G4)
(Plasmid #62683)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Total vector size (bp) 4970
  • Vector type
    Mammalian Expression, RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    amiR-eGFP419
  • gRNA/shRNA sequence
    TGTAGTTGTACTCCAGCTTGT

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pARD (pKD-G4) was a gift from Masato Ohtsuka (Addgene plasmid # 62683 ; http://n2t.net/addgene:62683 ; RRID:Addgene_62683)

  • For your References section:

    Assessment of Artificial MiRNA Architectures for Higher Knockdown Efficiencies without the Undesired Effects in Mice. Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato M, Ohtsuka M. PLoS One. 2015 Aug 18;10(8):e0135919. doi: 10.1371/journal.pone.0135919. eCollection 2015. PONE-D-14-52192 [pii] PubMed 26285215
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