pBER
(Plasmid
#62662)
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PurposeDonor vector for tdTomato knock-in via landing pad
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62662 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSMART-kan
- Total vector size (bp) 5990
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Vector typeCre/Lox
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nametdTomato
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Insert Size (bp)1431
Gene/Insert 2
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Gene/Insert namehygromycin
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Insert Size (bp)1020
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBER was a gift from Masato Ohtsuka (Addgene plasmid # 62662 ; http://n2t.net/addgene:62662 ; RRID:Addgene_62662) -
For your References section:
One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB. BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. 10.1186/s12864-015-1432-5 [pii] PubMed 25887549