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Addgene

pBDT
(Plasmid #62660)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS
  • Total vector size (bp) 12605
  • Vector type
    Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    717

Gene/Insert 2

  • Gene/Insert name
    neomycin
  • Insert Size (bp)
    792

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBDT was a gift from Masato Ohtsuka (Addgene plasmid # 62660 ; http://n2t.net/addgene:62660 ; RRID:Addgene_62660)

  • For your References section:

    One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB. BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. 10.1186/s12864-015-1432-5 [pii] PubMed 25887549
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