pAYC
(Plasmid
#62657)
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PurposeMammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAGGS
- Total vector size (bp) 5846
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiCre
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Insert Size (bp)1053
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAYC was a gift from Masato Ohtsuka (Addgene plasmid # 62657 ; http://n2t.net/addgene:62657 ; RRID:Addgene_62657) -
For your References section:
One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB. BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. 10.1186/s12864-015-1432-5 [pii] PubMed 25887549
Map uploaded by the depositor.
Map uploaded by the depositor.
