pKDsgRNA-p15 Citations (17)
Originally described in: The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.Reisch CR, Prather KL Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096. PubMed Journal
Articles Citing pKDsgRNA-p15
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Scarless Cas9 Assisted Recombineering (no-SCAR) in Escherichia coli, an Easy-to-Use System for Genome Editing. Reisch CR, Prather KLJ. Curr Protoc Mol Biol. 2017 Jan 5;117:31.8.1-31.8.20. doi: 10.1002/cpmb.29. PubMed |
vB_EcoS_IME347 a novel T1-like Escherichia coli bacteriophage. Li P, Lin H, Mi Z, Tong Y, Wang J. J Basic Microbiol. 2018 Nov;58(11):968-976. doi: 10.1002/jobm.201800271. Epub 2018 Aug 26. PubMed |
Comparative genomics analysis of pTEM-2262, an MDR plasmid from Citrobacter freundii, harboring two unclassified replicons. Li M, Li F, Mi Z, Zhao Y, Zhang X, Jiang Z, Pei G, Zhou L, Tong Y, Zhao B. Future Microbiol. 2018 Nov;13:1657-1668. doi: 10.2217/fmb-2018-0243. Epub 2018 Nov 30. PubMed |
Allosteric Feedback Inhibition Enables Robust Amino Acid Biosynthesis in E. coli by Enforcing Enzyme Overabundance. Sander T, Farke N, Diehl C, Kuntz M, Glatter T, Link H. Cell Syst. 2019 Jan 23;8(1):66-75.e8. doi: 10.1016/j.cels.2018.12.005. Epub 2019 Jan 9. PubMed |
Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing. Srinivas S, Hu Z, Cronan JE. Plasmid. 2019 May;103:53-62. doi: 10.1016/j.plasmid.2019.04.004. Epub 2019 Apr 29. PubMed |
Screening of Polyvalent Phage-Resistant Escherichia coli Strains Based on Phage Receptor Analysis. Li P, Lin H, Mi Z, Xing S, Tong Y, Wang J. Front Microbiol. 2019 Apr 18;10:850. doi: 10.3389/fmicb.2019.00850. eCollection 2019. PubMed |
A Combinatorial Approach to Optimize the Production of Curcuminoids From Tyrosine in Escherichia coli. Rodrigues JL, Gomes D, Rodrigues LR. Front Bioeng Biotechnol. 2020 Feb 7;8:59. doi: 10.3389/fbioe.2020.00059. eCollection 2020. PubMed |
Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits. Contreras-Llano LE, Meyer C, Liu Y, Sarker M, Lim S, Longo ML, Tan C. Nat Commun. 2020 Jun 19;11(1):3138. doi: 10.1038/s41467-020-16900-7. PubMed |
Sequence analysis and specificity of distinct types of menaquinone methyltransferases indicate the widespread potential of methylmenaquinone production in bacteria and archaea. Wilkens D, Meusinger R, Hein S, Simon J. Environ Microbiol. 2021 Mar;23(3):1407-1421. doi: 10.1111/1462-2920.15344. Epub 2020 Dec 15. PubMed |
Metabolome and proteome analyses reveal transcriptional misregulation in glycolysis of engineered E. coli. Wang CY, Lempp M, Farke N, Donati S, Glatter T, Link H. Nat Commun. 2021 Aug 13;12(1):4929. doi: 10.1038/s41467-021-25142-0. PubMed |
CRISPR/Cas9-mediated generation of auxotrophic Edwardsiella piscicida mutants and immunization in olive flounder (Paralichthys olivaceus). Lee EG, Kwak JS, Kim KH. Fish Shellfish Immunol. 2022 Mar;122:98-105. doi: 10.1016/j.fsi.2022.01.040. Epub 2022 Feb 1. PubMed |
Comprehensive Mutational Analysis of the Lasso Peptide Klebsidin. Hills E, Woodward TJ, Fields S, Brandsen BM. ACS Chem Biol. 2022 Apr 15;17(4):998-1010. doi: 10.1021/acschembio.2c00148. Epub 2022 Mar 22. PubMed |
Deletion of FRT-sites by no-SCAR recombineering in Escherichia coli.
Rangarajan AA, Yilmaz C, Schnetz K.
Microbiology (Reading). 2022 Apr;168(4). doi: 10.1099/mic.0.001173.
PubMed
Associated Plasmids |
Generating Single Nucleotide Point Mutations in E. coli with the No-SCAR System. Ellington AJ, Reisch CR. Methods Mol Biol. 2022;2479:119-133. doi: 10.1007/978-1-0716-2233-9_9. PubMed |
DELETE_Efficient and iterative retron-mediated in vivo recombineering in Escherichia coli. Ellington AJ, Reisch CR. Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 10.1093/synbio/ysac007. eCollection 2022. PubMed |
Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets. Chaves GL, Batista RS, Cunha JS, Oliveira DB, da Silva MR, Pisani GFD, Selistre-de-Araujo HS, Zangirolami TC, da Silva AJ. N Biotechnol. 2022 Dec 25;72:80-88. doi: 10.1016/j.nbt.2022.10.002. Epub 2022 Oct 19. PubMed |
Generation of bright autobioluminescent bacteria by chromosomal integration of the improved lux operon ilux2. Gregor C. Sci Rep. 2022 Nov 9;12(1):19039. doi: 10.1038/s41598-022-22068-5. PubMed |
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