pCas9-CR4
(Plasmid #62655)

• Purpose: Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR
• Depositing Lab: Kristala Prather
• Publication: Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.

Backbone

• Vector backbone: pdCas9-bacteria
• Total vector size (bp): 6770
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas9 nuclease

Resource Information

• Supplemental Documents:
  • noSCAR Protocol
• Addgene Notes:
  • Addgene diagnostic digest
• A portion of this plasmid was derived from a plasmid made by: Addgene, Stanley Qi lab
• Articles Citing this Plasmid:
  • 17 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected.