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pJexpress414_OmpA171_WTnoGirdles
(Plasmid #62653)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62653 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJexpress414
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4625
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OmpA_noGirdles
  • Species
    Synthetic
  • Insert Size (bp)
    642
  • Mutation
    Aromatic girdle residues mutated to Leu (W7, W15, Y43, F51, Y55, W57, Y85, F123, Y129, Y141, W143, but not Y168 or F170)
  • Promoter T7
  • Tags / Fusion Proteins
    • FlAsH (C terminal on insert)
    • 6His (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pTF 5′-CTCGAAAATAATAAAGGGAAAATCAG-3′
  • 3′ sequencing primer pTR 5′-TGGTAGTGTGGGGACTC-3′
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJexpress414_OmpA171_WTnoGirdles was a gift from Timothy Whitehead (Addgene plasmid # 62653 ; http://n2t.net/addgene:62653 ; RRID:Addgene_62653)

  • For your References section:

    Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Stapleton JA, Whitehead TA, Nanda V. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. 10.1073/pnas.1501836112 PubMed 26199411
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