pJexpress414_OmpA171_R4cons
(Plasmid
#62639)
-
PurposeConservative version of OmpA Redesign 4
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62639 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepJexpress
-
Backbone manufacturerDNA2.0
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 4625
-
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameOmpA_R4cons
-
SpeciesSynthetic
-
Insert Size (bp)627
-
Mutation25 mutations to lipid-facing surface
- Promoter T7
-
Tags
/ Fusion Proteins
- FlAsH (C terminal on insert)
- 6His (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pTF
- 3′ sequencing primer pTR (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJexpress414_OmpA171_R4cons was a gift from Timothy Whitehead (Addgene plasmid # 62639 ; http://n2t.net/addgene:62639 ; RRID:Addgene_62639) -
For your References section:
Computational redesign of the lipid-facing surface of the outer membrane protein OmpA. Stapleton JA, Whitehead TA, Nanda V. Proc Natl Acad Sci U S A. 2015 Jul 21. pii: 201501836. 10.1073/pnas.1501836112 PubMed 26199411