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Addgene

pcDNA3.1-CHRFAM7ADelta2bp-YFP
(Plasmid #62637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHRFAM7A
  • Alt name
    dhAlpha7
  • Alt name
    Human Alpha7 neuronal nicotinic acetylcholine receptor gene with partial duplication
  • Species
    H. sapiens (human)
  • Mutation
    there is a 2-bp deletion in exon 6 of CHRFAM7A and this causes a frameshift in translation, YFP inserted in M3-M4 loop
  • Entrez Gene
    CHRFAM7A (a.k.a. CHRNA7, CHRNA7-DR1, D-10, NACHRA7)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-CHRFAM7ADelta2bp-YFP was a gift from Henry Lester (Addgene plasmid # 62637 ; http://n2t.net/addgene:62637 ; RRID:Addgene_62637)

  • For your References section:

    The duplicated alpha7 subunits assemble and form functional nicotinic receptors with the full-length alpha7. Wang Y, Xiao C, Indersmitten T, Freedman R, Leonard S, Lester HA. J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 10.1074/jbc.M114.582858. Epub 2014 Jul 23. 10.1074/jbc.M114.582858 PubMed 25056953
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