pcDNA3.1-CHRFAM7ADelta2bp-mGFP
(Plasmid #62636)

• Purpose: mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop
• Depositing Lab: Henry Lester
• Publication: Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 10.1074/jbc.M114.582858. Epub 2014 Jul 23.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CHRFAM7A
• Alt name: dhAlpha7
• Alt name: Human Alpha7 neuronal nicotinic acetylcholine receptor gene with partial duplication
• Species: H. sapiens (human)
• Mutation: there is a 2-bp deletion in exon 6 of CHRFAM7A causing a frameshift in translation
• Entrez Gene: CHRFAM7A (a.k.a. CHRNA7, CHRNA7-DR1, D-10, NACHRA7)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEGFP inserted in M3-M4 loop

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry