pcDNA3.1-CHRNA7-mCherry
(Plasmid #62632)

• Purpose: mammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loop
• Depositing Lab: Henry Lester
• Publication: Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-63. doi: 10.1074/jbc.M114.582858. Epub 2014 Jul 23.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hAlpha7
• Alt name: Human Alpha7 neuronal nicotinic acetylcholine receptor gene
• Alt name: CHRNA7
• Species: H. sapiens (human)
• Mutation: mCherry inserted into M3-M4 loop
• Entrez Gene: CHRNA7 (a.k.a. CHRNA7-2, NACHRA7)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry