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LentiCRISPR_Hs_MBD3_ex2
(Plasmid #62623)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiCRISPR
  • Backbone manufacturer
    Zhang lab
  • Backbone size w/o insert (bp) 13450
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MBD3 gRNA
  • gRNA/shRNA sequence
    CTTGCCCGTGCGGAAGTCGA
  • Species
    H. sapiens (human)
  • Entrez Gene
    MBD3
  • Promoter EFS

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer hU6-F (for gRNA)
  • 3′ sequencing primer hSpCas9-R1 5'-CGCTCGTGCTTCTTATCCTC (for Cas9)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiCRISPR_Hs_MBD3_ex2 was a gift from Paul Wade (Addgene plasmid # 62623 ; http://n2t.net/addgene:62623 ; RRID:Addgene_62623)
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