pShuttle-CMV-F2A-T2A-Venus
(Plasmid #62621)

• Purpose: AdEasy Shuttle vector modified for multicistronic expression. Contains T2A, F2A and Venus tag
• Depositing Lab: Zheng-Xu Wang
• Publication: Modified AdEasy shuttle vectors for multicistronic expression (unpublished)

Backbone

• Vector backbone: pShuttle-CMV
• Backbone manufacturer: Agilent
• Total vector size (bp): 8360
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MCS with F2A and T2A
• Insert Size (bp): 165
• Promoter: CMV
• Tag / Fusion Protein:
  • Venus (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • Genbank
• A portion of this plasmid was derived from a plasmid made by: We cloned this.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry