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Addgene

pBGSA C5aR1
(Plasmid #62613)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62613 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBGSA
  • Backbone manufacturer
    Bruce Granger, PMID 8867788
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 8400
  • Modifications to backbone
    Modified from pBS SK+
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C5aR1
  • Alt name
    C5aR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1940
  • GenBank ID
    M62505
  • Entrez Gene
    C5AR1 (a.k.a. C5A, C5AR, C5R1, CD88)
  • Promoter SRalpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cttctaggcctgtacgg
  • 3′ sequencing primer tctagaactagtggatcc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBGSA C5aR1 was a gift from Heini Miettinen (Addgene plasmid # 62613 ; http://n2t.net/addgene:62613 ; RRID:Addgene_62613)
  • For your References section:

    Different endocytosis pathways of the C5a receptor and the N-formyl peptide receptor. Suvorova ES, Gripentrog JM, Miettinen HM. Traffic. 2005 Feb;6(2):100-15. 10.1111/j.1600-0854.2004.00256.x PubMed 15634211