pBGSA C5aR1-EGFP
(Plasmid #62612)

• Purpose: Constitutive expression of C5aR1-EGFP in mammalian cells
• Depositing Lab: Heini Miettinen
• Publication: Suvorova et al Traffic. 2005 Feb;6(2):100-15.

Backbone

• Vector backbone: pBGSA
• Backbone manufacturer: Bruce Granger, PMID 8867788
• Backbone size w/o insert (bp): 7200
• Total vector size (bp): 9140
• Modifications to backbone: Modified from pBS SK+
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: C5aR1
• Alt name: C5aR
• Species: H. sapiens (human)
• Insert Size (bp): 1940
• GenBank ID: M62505
• Entrez Gene: C5AR1 (a.k.a. C5A, C5AR, C5R1, CD88)
• Promoter: SRalpha
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: cttctaggcctgtacgg
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry