pBGSA FPR1-EGFP
(Plasmid #62604)

• Purpose: Constitutive expression of FPR1-EGFP in mammalian cells
• Depositing Lab: Heini Miettinen
• Publication: Gripentrog et al J Immunol. 2003 Sep 15;171(6):3187-93.

Backbone

• Vector backbone: pBGSA
• Backbone manufacturer: Bruce Granger, PMID 8867788
• Backbone size w/o insert (bp): 7200
• Total vector size (bp): 8520
• Modifications to backbone: Modified from pBS SK+
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FPR1
• Alt name: fmlp-R26
• Alt name: FPR1 (haplotype 3)
• Species: H. sapiens (human)
• Insert Size (bp): 2060
• GenBank ID: M60627
• Entrez Gene: FPR1 (a.k.a. FMLP, FPR)
• Promoter: SRalpha
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: cttctaggcctgtacgg
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Richard Ye, University of Illinois
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry