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pBGSA c-myc-FPR1
(Plasmid #62601)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62601 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBGSA
  • Backbone manufacturer
    Bruce Granger, PMID 8867788
  • Backbone size w/o insert (bp) 7200
  • Total vector size (bp) 8255
  • Modifications to backbone
    Modified from pBS SK+
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FPR1
  • Alt name
    fmlp-R26
  • Alt name
    FPR1 (haplotype 3)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1340
  • GenBank ID
    M60627
  • Entrez Gene
    FPR1 (a.k.a. FMLP, FPR)
  • Promoter SRalpha
  • Tag / Fusion Protein
    • c-myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer cttctaggcctgtacgg
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Richard Ye, University of Illinois

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBGSA c-myc-FPR1 was a gift from Heini Miettinen (Addgene plasmid # 62601 ; http://n2t.net/addgene:62601 ; RRID:Addgene_62601)

  • For your References section:

    Experimental evidence for lack of homodimerization of the G protein-coupled human N-formyl peptide receptor. Gripentrog JM, Kantele KP, Jesaitis AJ, Miettinen HM. J Immunol. 2003 Sep 15;171(6):3187-93. PubMed 12960347
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