pBGSA FPR1
(Plasmid #62600)

• Purpose: Constitutive expression of FPR1 in mammalian cells
• Depositing Lab: Heini Miettinen
• Publication: Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Backbone

• Vector backbone: pBGSA
• Backbone manufacturer: Bruce Granger, PMID 8867788
• Backbone size w/o insert (bp): 7200
• Total vector size (bp): 8520
• Modifications to backbone: Modified from pBS SK+
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FPR1
• Alt name: fmlp-R26
• Alt name: FPR1 (haplotype 3)
• Species: H. sapiens (human)
• Insert Size (bp): 1320
• GenBank ID: M60627
• Entrez Gene: FPR1 (a.k.a. FMLP, FPR)
• Promoter: SRalpha

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CTTCTAGGCCTGTACGG
• 3′ sequencing primer: TCTAGAACTAGTGGATCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Richard Ye, University of Illinois
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry