AI-CN443
(Plasmid #62593)

• Purpose: (Empty Backbone) TALEN array receiving vector with half-site NG RVD
• Depositing Lab: Joshua Grimley
• Publication: Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv164.

Backbone

• Vector backbone: JDS78
• Backbone size (bp): 6840
• Modifications to backbone: LacZ screening cassette flanked by BbsI sites for golden gate cloning
• Vector type: Mammalian Expression, TALEN ; T7
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3× FLAG (N terminal on backbone)
  • FOKI WT (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This material was constructed from JDS78 (AddGene Plasmid #32290). The TALEN insertion site was replaced with a LacZ selection cassette flanked by two BbsI sites. Other BbsI sites in the plasmid backbone were mutated.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry