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pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)
(Plasmid #62577)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIR-Report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 7700
  • Modifications to backbone
    To construct a WT SPRED1 reporter containing the putative miR-206 binding site, the 1.2-kb SPRED1 3′ UTR from clone BG167687 was excised by treatment with MluI and inserted into the same site of pMIR-REPORT.
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPRED1
  • Alt name
    Sprouty Related EVH1 domain containing 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • Entrez Gene
    SPRED1 (a.k.a. LGSS, NFLS, PPP1R147, hSpred1, spred-1)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Report-SPRED1-3'UTR (WT - miR-206 site) was a gift from Michael Ruppert (Addgene plasmid # 62577 ; http://n2t.net/addgene:62577 ; RRID:Addgene_62577)

  • For your References section:

    MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage KM, Salkeni MA, Ruppert JM. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. 10.1128/MCB.00480-14 PubMed 25202123
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