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pMIR-Report-RASA1-3'UTR (mut - miR-206 site)
(Plasmid #62576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIR-Report
  • Backbone manufacturer
    Ambion
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 7396
  • Modifications to backbone
    None. Cloning Strategy: To construct a WT RASA1 translational reporter, a 926-bp fragment of the RASA1 3′ UTR (from open biosystems, clone ID BC033015) was generated by sequential treatment with BamHI, Klenow fragment, and XbaI. This fragment was inserted into pMIR-REPORT vector that was prepared by sequential treatment with HindIII, Klenow fragment, and SpeI.
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RASA1
  • Alt name
    RAS p21 Activator (GTPase Activating Protein [GAP]) 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    926
  • Mutation
    miR-206 binding site mutated (ACATTCCA --> AACTCTCA)
  • Entrez Gene
    RASA1 (a.k.a. CM-AVM, CMAVM, CMAVM1, GAP, PKWS, RASA, RASGAP, p120, p120GAP, p120RASGAP)
  • Tag / Fusion Protein
    • None

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Report-RASA1-3'UTR (mut - miR-206 site) was a gift from Michael Ruppert (Addgene plasmid # 62576 ; http://n2t.net/addgene:62576 ; RRID:Addgene_62576)

  • For your References section:

    MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1. Sharma SB, Lin CC, Farrugia MK, McLaughlin SL, Ellis EJ, Brundage KM, Salkeni MA, Ruppert JM. Mol Cell Biol. 2014 Nov 15;34(22):4143-64. doi: 10.1128/MCB.00480-14. Epub 2014 Sep 8. 10.1128/MCB.00480-14 PubMed 25202123
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