huCaV2.2 (pSAD442-1)
(Plasmid #62574)

• Purpose: Human CaV2.2 expression in mammalian cells
• Depositing Lab: Diane Lipscombe
• Publication: Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993. Epub 2014 Oct 8.

Backbone

• Vector backbone: pcDNA6/V5-HisA
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 12200
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10F'
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CACNA1B
• Alt name: CaV2.2
• Alt name: huCaV2.2 (pSAD442-1)
• Species: H. sapiens (human)
• Insert Size (bp): 7100
• Entrez Gene: CACNA1B (a.k.a. BIII, CACNL1A5, CACNN, Cav2.2, DYT23, NEDNEH)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

contains alternate exons (+e10a, +18a, Δ19a, +e31a, +e37b and +e46)

Note that the human CaV2.2 insert contains the native stop codon. The V5 and His tags present in the pcDNA6/V5-HisA backbone are NOT in frame with CaV2.2.