GST-auxilin(547-910)
(Plasmid
#62572)
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PurposeCodes for a GST-auxilin fusion protein that is functional as a co-factor in hsc70-dependent clathrin coat disruption
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX4T-1
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Backbone manufacturerPharmacia
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe depositing laboratory recommends strain BL21 for protein expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAuxilin
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SpeciesB. taurus (bovine)
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Insert Size (bp)1200
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Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-auxilin(547-910) was a gift from Ernst Ungewickell (Addgene plasmid # 62572 ; http://n2t.net/addgene:62572 ; RRID:Addgene_62572) -
For your References section:
Mechanism of clathrin basket dissociation: separate functions of protein domains of the DnaJ homologue auxilin. Holstein SE, Ungewickell H, Ungewickell E. J Cell Biol. 1996 Nov;135(4):925-37. PubMed 8922377