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H6-auxilin
(Plasmid #62571)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62571 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    Qiagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing laboratory recommends strain BL21 for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Auxilin
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    3000
  • Mutation
    *see comment below
  • Tag / Fusion Protein
    • 6XHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer pBRforEco (5'-AATAGGCGTATCACGAGGC-3')
  • 3′ sequencing primer T0term-R (5'-GCGGCAACCGAGCGTTC-3)'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note: Addgene's quality control sequencing finds a T234I amino acid residue substitution in the bovine auxilin gene. This residue change is not thought to affect protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H6-auxilin was a gift from Ernst Ungewickell (Addgene plasmid # 62571 ; http://n2t.net/addgene:62571 ; RRID:Addgene_62571)

  • For your References section:

    Primary structure of the neuronal clathrin-associated protein auxilin and its expression in bacteria. Schroder S, Morris SA, Knorr R, Plessmann U, Weber K, Nguyen GV, Ungewickell E. Eur J Biochem. 1995 Mar 1;228(2):297-304. PubMed 7705342
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