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PurposeTetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSC101
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameGFP
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJKR-L-tetR was a gift from George Church (Addgene plasmid # 62562 ; http://n2t.net/addgene:62562 ; RRID:Addgene_62562) -
For your References section:
Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. 10.1093/nar/gkv616 PubMed 26152303