pJKR-H-cdaR
(Plasmid
#62557)
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PurposeGlucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62557 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC
- Total vector size (bp) 5361
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namebeta lactamase
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Insert Size (bp)861
Gene/Insert 2
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Gene/Insert namesfGFP
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Insert Size (bp)714
Gene/Insert 3
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Gene/Insert nameCdaR
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Insert Size (bp)1158
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJKR-H-cdaR was a gift from George Church (Addgene plasmid # 62557 ; http://n2t.net/addgene:62557 ; RRID:Addgene_62557) -
For your References section:
Synthetic biosensors for precise gene control and real-time monitoring of metabolites. Rogers JK, Guzman CD, Taylor ND, Raman S, Anderson K, Church GM. Nucleic Acids Res. 2015 Jul 7. pii: gkv616. 10.1093/nar/gkv616 PubMed 26152303