ANO6-Plvx-mCherry-c1
(Plasmid #62554)

• Purpose: Full length mouse ANO6 tagged with mCherry on the C-terminus
• Depositing Lab: Renzhi Han
• Publication: Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi: 10.1371/journal.pone.0092749. eCollection 2014.

Backbone

• Vector backbone: pLVX-mCherry-C1
• Backbone size w/o insert (bp): 7900
• Total vector size (bp): 11500
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mouse ANO6
• Alt name: Anoctamin 6
• Species: M. musculus (mouse)
• Entrez Gene: Ano6 (a.k.a. 2900059G15Rik, AA407480, AW554778, F730003B03Rik, Tmem16f)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV FORWARD
• 3′ sequencing primer: mCherry-R (5'-TTGGTCACCTTCAGCTTGG-3')

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry