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pKCcas9dO Citations (4)

Originally described in: One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces.
Huang H, Zheng G, Jiang W, Hu H, Lu Y Acta Biochim Biophys Sin (Shanghai). 2015 Apr;47(4):231-43. doi: 10.1093/abbs/gmv007. Epub 2015 Mar 3.
PubMed Journal

Articles Citing pKCcas9dO

Articles
The pSG5-based thermosensitive vector family for genome editing and gene expression in actinomycetes. Muth G. Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 10.1007/s00253-018-9334-5. Epub 2018 Sep 6. PubMed

Associated Plasmids

Characterization of a LAL-type regulator NemR in nemadectin biosynthesis and its application for increasing nemadectin production in Streptomyces cyaneogriseus. Li C, He H, Wang J, Liu H, Wang H, Zhu Y, Wang X, Zhang Y, Xiang W. Sci China Life Sci. 2019 Mar;62(3):394-405. doi: 10.1007/s11427-018-9442-9. Epub 2019 Jan 15. PubMed
CRISPR/Cas9-mediated genome editing of Shewanella oneidensis MR-1 using a broad host-range pBBR1-based plasmid. Suzuki Y, Kouzuma A, Watanabe K. J Gen Appl Microbiol. 2020 Apr 13;66(1):41-45. doi: 10.2323/jgam.2019.04.007. Epub 2019 Aug 23. PubMed
Elucidation of the Activation Pathways of ScyA1/ScyR1, an Aco/ArpA-Like System That Regulates the Expression of Nemadectin and Other Secondary Metabolic Biosynthetic Genes. Liu H, Zhang Y, Li S, Wang J, Wang X, Xiang W. Front Bioeng Biotechnol. 2020 Nov 3;8:589730. doi: 10.3389/fbioe.2020.589730. eCollection 2020. PubMed

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