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PurposeBroad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKK233-2
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Backbone manufacturerPharmacia
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
- Promoter trc
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13pUC-Rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Nagant, et al. for an example of use of this plasmid (Microbiologyopen. 2013 Apr;2(2):318-25)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMF440 was a gift from Michael Franklin (Addgene plasmid # 62550 ; http://n2t.net/addgene:62550 ; RRID:Addgene_62550)
