Skip to main content
Addgene

pT2/LTR7-GFP
(Plasmid #62541)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pT2/BH
  • Backbone size w/o insert (bp) 3373
  • Total vector size (bp) 5644
  • Vector type
    Mammalian Expression ; Sleeping Beauty transposon

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LTR7-GFP
  • Insert Size (bp)
    2000
  • Promoter LTR7

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GCGTGAATTCATGCTGCGAGATGGGAAACA
  • 3′ sequencing primer AATCGCTAGCGGGTGAAGGAGAAGGGGTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2/LTR7-GFP was a gift from Zsuzsanna Izsvak (Addgene plasmid # 62541 ; http://n2t.net/addgene:62541 ; RRID:Addgene_62541)

  • For your References section:

    Primate-specific endogenous retrovirus-driven transcription defines naive-like stem cells. Wang J, Xie G, Singh M, Ghanbarian AT, Rasko T, Szvetnik A, Cai H, Besser D, Prigione A, Fuchs NV, Schumann GG, Chen W, Lorincz MC, Ivics Z, Hurst LD, Izsvak Z. Nature. 2014 Dec 18;516(7531):405-9. doi: 10.1038/nature13804. Epub 2014 Oct 15. 10.1038/nature13804 PubMed 25317556
Related items:
Commonly requested with: