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GFP-Drosha270-1374S300/302A
(Plasmid #62533)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62533 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8012
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    truncated human Drosha mutant
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3312
  • Mutation
    deleted amino acids 1-269 and changed serines 300 & 302 to alanines
  • Entrez Gene
    DROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer GACGAGCTGTACAAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Drosha270-1374S300/302A was a gift from Bharat Ramratnam (Addgene plasmid # 62533 ; http://n2t.net/addgene:62533 ; RRID:Addgene_62533)

  • For your References section:

    Phosphorylation of the RNase III enzyme Drosha at Serine300 or Serine302 is required for its nuclear localization. Tang X, Zhang Y, Tucker L, Ramratnam B. Nucleic Acids Res. 2010 Oct;38(19):6610-9. doi: 10.1093/nar/gkq547. Epub 2010 Jun 16. 10.1093/nar/gkq547 PubMed 20554852
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