GFP-DroshaS300/302A
(Plasmid
#62530)
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PurposeSerines 300 and 302 of Drosha are mutated to alanines
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62530 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 8822
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Drosha with serines 300 and 302 changed alanines
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SpeciesH. sapiens (human)
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Insert Size (bp)4122
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Mutationchanged serines 300 and 302 to alanines
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Entrez GeneDROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer GACGAGCTGTACAAG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Drosha contains H278R. Depositor states that this mutation should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-DroshaS300/302A was a gift from Bharat Ramratnam (Addgene plasmid # 62530 ; http://n2t.net/addgene:62530 ; RRID:Addgene_62530) -
For your References section:
Phosphorylation of the RNase III enzyme Drosha at Serine300 or Serine302 is required for its nuclear localization. Tang X, Zhang Y, Tucker L, Ramratnam B. Nucleic Acids Res. 2010 Oct;38(19):6610-9. doi: 10.1093/nar/gkq547. Epub 2010 Jun 16. 10.1093/nar/gkq547 PubMed 20554852