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Addgene

GFP-DroshaS302A
(Plasmid #62527)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62527 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8822
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Drosha with serine 302 changed to alanine
  • Species
    H. sapiens (human)
  • Mutation
    changed serine 302 to alanine
  • Entrez Gene
    DROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer GACGAGCTGTACAAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Drosha contains H278R mutation. Depositor states this mutation should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-DroshaS302A was a gift from Bharat Ramratnam (Addgene plasmid # 62527 ; http://n2t.net/addgene:62527 ; RRID:Addgene_62527)

  • For your References section:

    Phosphorylation of the RNase III enzyme Drosha at Serine300 or Serine302 is required for its nuclear localization. Tang X, Zhang Y, Tucker L, Ramratnam B. Nucleic Acids Res. 2010 Oct;38(19):6610-9. doi: 10.1093/nar/gkq547. Epub 2010 Jun 16. 10.1093/nar/gkq547 PubMed 20554852
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