GFP-Drosha1-390
(Plasmid #62521)

• Purpose: C-terminal aa391-1374 of Drosha is deleted
• Depositing Lab: Bharat Ramratnam
• Publication: Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-9. doi: 10.1093/nar/gkq547. Epub 2010 Jun 16.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5870
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Drosha with aa 391-1374 deleted
• Species: H. sapiens (human)
• Insert Size (bp): 1170
• Mutation: deleted amino acids 391-1374
• Entrez Gene: DROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: GACGAGCTGTACAAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct aligns with Drosha isoform-2 (NP_001093882.1) up to amino acid 353 or amino acid 390 of isoform-1 (NP_037367; with deletion of residues 316-352). Drosha 1 also contains S195G and H353S mutations. Depositor states that these mutations should not affect function.