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Addgene

GFP-DroshaK382R
(Plasmid #62519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 8822
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Drosha with lysine 382 changed to arginine
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4122
  • Mutation
    changed lysine 383 to arginine
  • Entrez Gene
    DROSHA (a.k.a. ETOHI2, HSA242976, RANSE3L, RN3, RNASE3L, RNASEN)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer GACGAGCTGTACAAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Drosha contains M2V, K214R and K380R. Depositor states that these mutation should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-DroshaK382R was a gift from Bharat Ramratnam (Addgene plasmid # 62519 ; http://n2t.net/addgene:62519 ; RRID:Addgene_62519)

  • For your References section:

    Acetylation of drosha on the N-terminus inhibits its degradation by ubiquitination. Tang X, Wen S, Zheng D, Tucker L, Cao L, Pantazatos D, Moss SF, Ramratnam B. PLoS One. 2013 Aug 29;8(8):e72503. doi: 10.1371/journal.pone.0072503. eCollection 2013. PONE-D-13-12890 [pii] PubMed 24009686
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