pAAV-UbC-tdTomato
(Plasmid #62516)

• Purpose: Expresses tdTomato under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV. Optimal for tracing axons in tissue clearing procedures
• Depositing Labs: Jae Lee, Pantelis Tsoulfas
• Publication: Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.

Backbone

• Vector backbone: pAAV-SIBR-anti_I
• Backbone manufacturer: Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
• Backbone size w/o insert (bp): 5212
• Total vector size (bp): 6661
• Modifications to backbone: The anti-luciferase SIBR and eGFP sequence was deleted from the original backbone and new polylinker was created in the place of eGFP.
• Vector type: Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Species: Was optimized on Human codon usage
• Insert Size (bp): 1449
• Promoter: hUbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pCAG_F_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The tdTomato was constructed synthetically based on the Human codon usage.