3I91
(Plasmid #62514)

• Purpose: Bacterial expression plasmid for use in structural determination.
• Depositing Lab: Cheryl Arrowsmith
• Publication: Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. doi: 10.1074/jbc.M110.191411. Epub 2010 Nov 3.

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: SGC (Addgene Plasmid # 26096)
• Backbone size w/o insert (bp): 7325
• Total vector size (bp): 7487
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CBX8
• Alt name: chromobox homolog 8
• Alt name: PDB IDs: 3I91, 5EQ0
• Species: H. sapiens (human)
• Insert Size (bp): 162
• GenBank ID:
• Entrez Gene: CBX8 (a.k.a. PC3, RC1)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (N terminal on backbone)
  • TEV cleavage (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, please see:
http://www.thesgc.org/structures/3i91/
http://www.thesgc.org/structures/5EQ0/