3I91
(Plasmid
#62514)
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PurposeBacterial expression plasmid for use in structural determination.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 62514 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene Plasmid # 26096)
- Backbone size w/o insert (bp) 7325
- Total vector size (bp) 7487
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCBX8
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Alt namechromobox homolog 8
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Alt namePDB IDs: 3I91, 5EQ0
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SpeciesH. sapiens (human)
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Insert Size (bp)162
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GenBank ID
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Entrez GeneCBX8 (a.k.a. PC3, RC1)
- Promoter T7
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Tags
/ Fusion Proteins
- 6xHis (N terminal on backbone)
- TEV cleavage (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, please see:
http://www.thesgc.org/structures/3i91/
http://www.thesgc.org/structures/5EQ0/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3I91 was a gift from Cheryl Arrowsmith (Addgene plasmid # 62514 ; http://n2t.net/addgene:62514 ; RRID:Addgene_62514) -
For your References section:
Recognition and specificity determinants of the human cbx chromodomains. Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li Z, Vedadi M, Schapira M, Min J, Arrowsmith CH. J Biol Chem. 2011 Jan 7;286(1):521-9. doi: 10.1074/jbc.M110.191411. Epub 2010 Nov 3. 10.1074/jbc.M110.191411 PubMed 21047797