pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S
(Plasmid #62460)

• Purpose: expresses C151S and C288S double mutant human Keap1 protein
• Depositing Lab: Yimon Aye
• Publication: Parvez et al J Am Chem Soc. 2014 Dec 31.

Backbone

• Vector backbone: pMIR
• Backbone size w/o insert (bp): 6470
• Total vector size (bp): 8370
• Modifications to backbone: Contains TRAF6 in the 3'-UTR
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Keap1
• Species: H. sapiens (human)
• Insert Size (bp): 2859
• Mutation: Changed Cysteines 151 and 288 of Keap1 to respective serine residues
• GenBank ID: NM_203500.1
• Entrez Gene: KEAP1 (a.k.a. INrf2, KLHL19)
• Promoter: CMV
• Tag / Fusion Protein:
  • His-Halo-Tev (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry