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pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S
(Plasmid #62459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 62459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMIR
  • Backbone size w/o insert (bp) 6470
  • Total vector size (bp) 8370
  • Modifications to backbone
    Contains TRAF6 in the 3'-UTR
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keap1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2859
  • Mutation
    Changed Cysteine 288 of Keap1 to serine
  • GenBank ID
    NM_203500.1
  • Entrez Gene
    KEAP1 (a.k.a. INrf2, KLHL19)
  • Promoter CMV
  • Tag / Fusion Protein
    • His-Halo-Tev (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S was a gift from Yimon Aye (Addgene plasmid # 62459 ; http://n2t.net/addgene:62459 ; RRID:Addgene_62459)

  • For your References section:

    Substoichiometric Hydroxynonenylation of a Single Protein Recapitulates Whole-Cell-Stimulated Antioxidant Response. Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y. J Am Chem Soc. 2014 Dec 31. 10.1021/ja5084249 PubMed 25544059
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