pMIR-Dsred-IRES-His Keap
(Plasmid
#62457)
-
Purposeexpresses his tagged human Keap1 protein
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 62457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMIR
- Backbone size w/o insert (bp) 6470
- Total vector size (bp) 8370
-
Modifications to backboneContains TRAF6 in the 3'-UTR
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKeap1
-
Alt nameKelch-like ECH associated protein 1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1900
-
GenBank IDNM_203500.1
-
Entrez GeneKEAP1 (a.k.a. INrf2, KLHL19)
- Promoter CMV
-
Tag
/ Fusion Protein
- HisTag (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMV fwd (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMIR-Dsred-IRES-His Keap was a gift from Yimon Aye (Addgene plasmid # 62457 ; http://n2t.net/addgene:62457 ; RRID:Addgene_62457) -
For your References section:
Substoichiometric Hydroxynonenylation of a Single Protein Recapitulates Whole-Cell-Stimulated Antioxidant Response. Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y. J Am Chem Soc. 2014 Dec 31. 10.1021/ja5084249 PubMed 25544059