pet28a-His6-Halo-Tev-Keap1
(Plasmid
#62456)
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PurposeHis6-halo tagged human Keap1 protein for E.coli expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepet28a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5369
- Total vector size (bp) 8200
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKeap1
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SpeciesH. sapiens (human)
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Insert Size (bp)2800
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GenBank IDNM_203500.1
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Entrez GeneKEAP1 (a.k.a. INrf2, KLHL19)
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Tag
/ Fusion Protein
- His-Halo-Tev (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer T7 Rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pet28a-His6-Halo-Tev-Keap1 was a gift from Yimon Aye (Addgene plasmid # 62456 ; http://n2t.net/addgene:62456 ; RRID:Addgene_62456) -
For your References section:
Substoichiometric Hydroxynonenylation of a Single Protein Recapitulates Whole-Cell-Stimulated Antioxidant Response. Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y. J Am Chem Soc. 2014 Dec 31. 10.1021/ja5084249 PubMed 25544059
