pet28a-His6-Halo-TEV-Nrf2
(Plasmid #62455)

• Purpose: Halo-His6 tagged Nrf2 for E.coli expression
• Depositing Lab: Yimon Aye
• Publication: Parvez et al J Am Chem Soc. 2014 Dec 31.

Backbone

• Vector backbone: pet28a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7247
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrf2
• Alt name: Nuclear factor, erythroid 2 like 2
• Species: H. sapiens (human)
• Insert Size (bp): 1878
• GenBank ID: NM_006164.4
• Entrez Gene: NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
• Tag / Fusion Protein:
  • His6-Halo-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: T7 Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry