pet28a-His6-Keap1
(Plasmid #62454)

• Purpose: His6 tagged Keap1 for E.coli expression
• Depositing Lab: Yimon Aye
• Publication: Parvez et al J Am Chem Soc. 2014 Dec 31.

Backbone

• Vector backbone: pet28a
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Keap1
• Alt name: Kelch-like ECH associated protein 1
• Species: H. sapiens (human)
• Insert Size (bp): 1935
• GenBank ID: NM_203500.1
• Entrez Gene: KEAP1 (a.k.a. INrf2, KLHL19)
• Tag / Fusion Protein:
  • His-Tag (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: T7 Rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry