rtTA and TagBFP cassettes
(Plasmid
#62452)
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PurposeMXS_chaining vector with 2 cassettes
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 62452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneMXS_chaining vector
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Backbone manufacturerNeveu lab, Addgene plasmid #62394
- Backbone size w/o insert (bp) 1945
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCMV::NLS-TagBFPx3-bGHpA
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SpeciesSynthetic
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer TTACCGCCTTTGAGTGAG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameCMV::rtTA3-bGHpA
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rtTA and TagBFP cassettes was a gift from Pierre Neveu (Addgene plasmid # 62452 ; http://n2t.net/addgene:62452 ; RRID:Addgene_62452) -
For your References section:
MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. Sladitschek HL, Neveu PA. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PONE-D-15-01385 [pii] PubMed 25909630
