MXS_CMV::CreERT2-bGHpA
(Plasmid #62443)

• Purpose: MXS_chaining vector with CMV::CreERT2-bGHpA
• Depositing Lab: Pierre Neveu
• Publication: Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.

Backbone

• Vector backbone: MXS_chaining vector
• Backbone manufacturer: Neveu lab, Addgene plasmid #62394
• Backbone size w/o insert (bp): 1945
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
• Species: Synthetic
• Insert Size (bp): 2863

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: TTACCGCCTTTGAGTGAG
• 3′ sequencing primer: TTGTCTCATGAGCGGATAC

Resource Information

• Supplemental Documents:
  • Annotated Genbank file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry