MXS_rtTA3
(Plasmid
#62428)
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PurposeMXS_chaining vector with rtTA3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 62428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMXS_chaining vector
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Backbone manufacturerNeveu lab, Addgene plasmid #62394
- Backbone size w/o insert (bp) 1945
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameReverse tetracycline transactivator
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SpeciesSynthetic
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Insert Size (bp)795
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer TTACCGCCTTTGAGTGAG
- 3′ sequencing primer TTGTCTCATGAGCGGATAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MXS_rtTA3 was a gift from Pierre Neveu (Addgene plasmid # 62428 ; http://n2t.net/addgene:62428 ; RRID:Addgene_62428) -
For your References section:
MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. Sladitschek HL, Neveu PA. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PONE-D-15-01385 [pii] PubMed 25909630